Did not fix it, however, I figured it.
Sometimes, I do not know yet why, Agisoft lies in a state where the "Adjusted Calibration" tab is greyed. Somehow, it did yield always 27.505... although the tab was not active and accessible...
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My main goal was also actually not to reset anything, but watch the k4, b1 and b2 parameters to check, if "Optimize cameras" has been run by the user before, or if this step is still open.
Thanks still for the answer.
PS: meta did contain some stuff, however, nothing related to the Calibration. Its just a dict with some sensor info about its name...