if you already have the 28-135 there is no need to buy the prime 35mm for your tests, as you are anyway going to use a small aperture (f8-f11) to maximize depth of field. The sharpness advantage of primes diminishes as you close down the aperture. A prime also has less distortions which would lead to a better reconstruction but what you have is a good start
You'll just have to make sure you dont change focal lengths between shots.
Also considering the 1.6 crop factor you could also use the lower end of your zoom range (easier to check that you have a fixed focal lenghth for all shots for uniformity), as 28x1.6=~45mm