Hello varadg,
Estimation of the interior calibration parameters is just a substep of Align Photos operation, apart from it the procedure also includes feature point detection, image matching and exterior parameters estimation.
In principle, you can run rather quick test - align the image set as usual, letting PhotoScan to estimate the calibration parameters, then export the adjusted values, load them to the Initial tab and fix the calibration, then re-start the processing using the same parameters and compare the processing time.