Hello VegetableSausage,
You need to mask out the static background to be able to align all images in the single chunk. In principle, you can create the rough mesh models in the separate chunks for "top" and "bottom" subsets, then generate masks from model and then drag and drop all images from both chunks to the single one and run the alignment using "Constrain features by mask" option.
But of course, like James has already mentioned, it's necessary to have good overlap between the images from the different subsets.