Hi Jumboo,
If you are using a non-metric camera (e.g., standard DSLR, mirrorless camera or a DJI drone with built in camera) then it is NOT recommended to perform a manual camera calibration but using ground control points and the self calibration.
Note that you could also do a manual calibration in PhotoScan (TOOLS > LENS) in case you have a metric camera. No need for Matlab or openCV.
Regards,
SAV